PREDICTIVE VALUE OF CD34+ CELLS IN BLOOD OF PATIENT/DONOR BEFORE HEMATOPOIETIC STEM CELLS COLLECTION BY LEUKAPHERESIS
Abstract
Background. In the study we tried to define a predictive value of the circulating CD34+ cells in patients/ donors blood for estimation of the hematopoietic stem cells (HSC) collection efficacy determine the optimal time to initiate the collection by leukapheresis procedure.
Methods. We retrospectively analyzed 75 collections of HSC using the Amicus cell separator in 39 patients and 15 donors. Circulating CD34+cell counts in patients/donors were compared to the achieved CD34+ cell yields to determine its predictive value for the collection of a targeted yield of > 2 × 106 CD34+ cells/kg body weight of patient.
Results. The results of cell counts confirmed that mobilization regimens were successful and HSC collections efficient. High correlation coefficient (r = 0.82) between the number of circulating CD34+ cells before collection and CD34+ cell yield/kg of patient’s body weight was statistically significant (p < 0.05). With ROC analysis we determined the cut-off value 42 × 106/l CD34+ cell counts in the blood of patients/donors before collection that had a positive predictive value 87% and a negative predictive value 91.6%.
Conclusions. Analysis showed that the number of circulating CD34+ cells before the procedure express a very high predictive value and can be used for determining the optimal time to initiate collection of HSC by leukapheresis.
Downloads
References
Champlin RE, Schmitz N, Horowitz MM, et al. Blood and stem cells compared with bone marrow as a source of hematopoietic cells for allogeneic transplantation. Blood 2000; 95: 3702–9.
Wats MJ, Linch DC. Peripheral blood stem cell transplantation.Vox Sang 1997; 73: 135–42.
Gillespie TW, Hillyer CD. Peripheral blood progenitor cells for marrow reconstitution: mobilization and collection strategies. Transfusion 1996; 36: 611–24.
Bender JG,To LB, Wikiams S, Schwartzberg LS. Defining a therapeutic dose of peripheral blood stem cells. J Hematother 1992; 1: 329–41.
Ketterer N, Salles G, Raba M et al. High CD34+ cell counts decrease hematologic toxicity of autologous peripheral blood progenitor cell transplantation. Blood 1998; 91: 3184–55.
Passos-Coelho JR, Braine HG, Davis JM et al. Predictive factors for peripheralblood-progenitor-cell collections using a single large-volume leukapheresis after cyclophosphamide and granulocyte-macrophage colony-stimulating factor mobilization. J Clin Oncol 1995; 13: 705–14.
Humpe A. Riggert J, Meineke I et al. A cell kinetic model of CD34+ cell mobilization and harvest: development of predictive algorithm for CD34+ cell yield in PBPC collection. Transfusion 2000; 40: 1363–70.
Passos-Coelho JR, Braine HG, Davis JM et al. Predictive factors for peripheralblood-progenitor-cell collections using a single large-volume leukapheresis after cyclophosphamide and granulocyte-macrophage colony-stimulating factor mobilization. J Clin Oncol 1995; 13: 705–14.
Nadler SB, Hidalgo JU, Bloch T. Prediction of blood volume in normal human adults. Surgery 1962; 51: 224–323.
Sutherland RD, Anderson L, Keeney M et al. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. J Hematother 1996; 5: 213–26.
Rowley SD, Prather K, Bui KT et al. Collection of peripheral blood progenitor cells with an automated leukapheresis system. Transfusion 1999; 39: 1200–6.
Witt V, Fischmeister G, Scharner D et al. Collection efficiencies of MNC subpopulations during autologous CD34+ peripheral blood progenitor cell (PBPC) harvest in small children and adolescents. J Clin Apheresis 2001; 16: 161–8.
Bensinger W, Appelbaum F, Rowley S et al. Factors that influence collection and engraftment of autologous peripheral-blood stem cells. J Clin Oncol 1995; 13: 2547–55.
Scots R, Van Riet I, Damiaens S et al. The absolute number of circulating CD34+ cells predicts the number of hematopoietic stem cells that can be collected by apheresis. Bone Marrow Transplant 1996; 17: 509–15.
Yu J, Leisenring W, Bensinger WI et al. The predictive value of white cell or CD34+ cell count in the peripheral blood for timing apheresis and maximizing yield. Transfusion 1999; 39: 442–50.
Snyder EL, O’Donnell L, Dengler TJ et al. Ex vivo evaluation of PBMNCs collected with a new cell separator. Transfusion 2001; 41: 940–1.
Möhle R, Murea S, Pförsich M et al. Estimation of progenitor cell yield in a leukapheresis product by previous measurement of CD34+ cells in peripheral blood. Vox Sanguinis 1996; 71: 90–6.
Brown RA, Adkins D, Goodnough LT et al. Factors that influence the collection and engraftment of allogeneic peripheral-blood stem cells in patients with hematologic malignacies. J Clin Onc 1997; 15 (9): 3067–74.
The Author transfers to the Publisher (Zdravniški vestnik/Slovenian Medical Journal) all economic copyrights following form Article 22 of the Slovene Copyright and Related Rights Act (ZASP), including the right of reproduction, the right of distribution, the rental right, the right of public performance, the right of public transmission, the right of public communication by means of phonograms and videograms, the right of public presentation, the right of broadcasting, the right of rebroadcasting, the right of secondary broadcasting, the right of communication to the public, the right of transformation, the right of audiovisual adaptation and all other rights of the author according to ZASP.
The aforementioned rights are transferred non-exclusively, for an unlimited number of editions, for the term of the statutory
The Author can make use of his work himself or transfer subjective rights to others only after 3 months from date of first publishing in the journal Zdravniški vestnik/Slovenian Medical Journal.
The Publisher (Zdravniški vestnik/Slovenian Medical Journal) has the right to transfer the rights, acquired parties without explicit consent of the Author.
The Author consents that the Article be published under the Creative Commons BY-NC 4.0 (attribution-non-commercial) or comparable licence.