EPSTEIN-BARR VIRUS INFECTIONS – AVIDITY TEST FOR IgG ANTIBODIES
Abstract
Background. We wish to introduce specific IgG avidity test as a supplementary assay in serological screening for Epstein-Barr virus infection if the status of patient cannot be resolved from a single serum sample with routine testing.
Methods. Avidity of IgG antibodies was determined in sera of 57 patients with different stage of Epstein-Barr virus infection. Enzyme-immuno assay was used with a short incubation of 6-molar urea included in the procedure. Urea should remove low avidity antibodies. Avidity was expressed as the avidity index. Avidity testing with commercial kit was done as well.
Results. Low avidity index was found for IgG antibodies of acute phase sera and high for those of past infection, recent infection and reactivation of endogenic virus.
Conclusions. Avidity test for IgG antibodies might be supplementary assay to prove acute infection but also to resolve some other clinical states related to Epstein-Barr virus.
Downloads
References
Okano M. Epstein-Barr virus infection and its role in the expanding spectrum of human diseases. Acta Paediatr 1998; 87: 11–8.
Faulkner GC, Krajewski AS, Crawford DH. The ins and outs of EBV infections. Trends in Microbiol 2000; 8: 185–9.
Marin J. Dokazovanje okužb, ki jih povzroŁa virus Epstein-Barr; encimsko imunski test namesto posredne imunofluorescence. Zdrav Vestn 1995; 64: 13–4.
Anderson A, Vetter V, Kreutzer L, Bauer G. Avidities of IgG directed against viral capsid antigen or early antigen: useful markers for significant EpsteinBarr virus serology. J Med Virol 1994; 43: 238–44.
Weissbrich B. The use of semi-automated EBV IgG avidity determination for the diagnosis of infectious mononucleosis. J Med Virol 1998; 54: 145–53.
Strašek K. Diagnostika okužb z virusom Epstein-Barr – ugotavljanje avidnosti IgG protiteles. Diplomska naloga. Univerza v Ljubljani, Biotehniška fakulteta, Enota medoddelŁnega študija mikrobiologije 2000: 1–61.
Schubert J, Zens W, Weissbrich B. Comparative evaluation of the use of immunoblots and of IgG avidity assays as confirmatory tests for the diagnosis of acute EBV infections. J Clin Virol 1998; 11: 161–72.
Gray JJ. Avidity of EBV VCA-specific IgG antibodies: distinction between recent primary infection, past infection and reactivation. J Virol Methods 1995; 52: 95–104.
The Author transfers to the Publisher (Zdravniški vestnik/Slovenian Medical Journal) all economic copyrights following form Article 22 of the Slovene Copyright and Related Rights Act (ZASP), including the right of reproduction, the right of distribution, the rental right, the right of public performance, the right of public transmission, the right of public communication by means of phonograms and videograms, the right of public presentation, the right of broadcasting, the right of rebroadcasting, the right of secondary broadcasting, the right of communication to the public, the right of transformation, the right of audiovisual adaptation and all other rights of the author according to ZASP.
The aforementioned rights are transferred non-exclusively, for an unlimited number of editions, for the term of the statutory
The Author can make use of his work himself or transfer subjective rights to others only after 3 months from date of first publishing in the journal Zdravniški vestnik/Slovenian Medical Journal.
The Publisher (Zdravniški vestnik/Slovenian Medical Journal) has the right to transfer the rights, acquired parties without explicit consent of the Author.
The Author consents that the Article be published under the Creative Commons BY-NC 4.0 (attribution-non-commercial) or comparable licence.