FISH panels for pediatric acute leukemias
Abstract
Background: Conventional cytogenetics is the gold standard in the diagnostic work up of acute leukemia patients. Due to a low mitotic index, lack of sample, and poor chromosome morphology, fluorescence in situ hybridization (FISH) is widely used as a complementary method to conventional cytogenetics. Additionally, some cryptic chromosomal rearrangements, which are crucial for stratification of patients, can be detected only by FISH. Different FISH panels are proposed to obtain comprehensive cytogenetic information. Methods: Recurrent chromosomal abnormalities were detected using commercially available FISH DNA probes. Results: In 85 % of pediatric AL patients conventional cytogenetics was successfully completed, revealing chromosomal rearrangements in half of them. We introduce an algorithm for inclusion of FISH DNA probes into panel, which is based on cytomorphology and immunophenotype of leukemic blasts. Using these panels, a particular cytogenetic marker was determined in 70 % of tested samples. The difference is even more prominent in B-ALL where conventional cytogenetics is less efficient. The frequency of a particular recurrent aberration is comparable with literature data in general, although some discrepancies were observed. Besides the detection of targeted rearrangements, FISH panels frequently show additional chromosomal changes with defined diagnostic and prognostic significance. Conclusions: The proposed algorithm for inclusion of DNA-probes into FISH panels is particularly efficient in children with B-ALL. In addition, presented results confirm this approach as an appropriate in different leukemias.Downloads
The Author transfers to the Publisher (Zdravniški vestnik/Slovenian Medical Journal) all economic copyrights following form Article 22 of the Slovene Copyright and Related Rights Act (ZASP), including the right of reproduction, the right of distribution, the rental right, the right of public performance, the right of public transmission, the right of public communication by means of phonograms and videograms, the right of public presentation, the right of broadcasting, the right of rebroadcasting, the right of secondary broadcasting, the right of communication to the public, the right of transformation, the right of audiovisual adaptation and all other rights of the author according to ZASP.
The aforementioned rights are transferred non-exclusively, for an unlimited number of editions, for the term of the statutory
The Author can make use of his work himself or transfer subjective rights to others only after 3 months from date of first publishing in the journal Zdravniški vestnik/Slovenian Medical Journal.
The Publisher (Zdravniški vestnik/Slovenian Medical Journal) has the right to transfer the rights, acquired parties without explicit consent of the Author.
The Author consents that the Article be published under the Creative Commons BY-NC 4.0 (attribution-non-commercial) or comparable licence.
