ANALYSIS OF CORD BLOOD UNITS DONATED TO THE SLOVENIAN CORD BLOOD BANK
Abstract
Background Umbilical cord blood (CB) as well as bone marrow has been used to treat a variety of haematopoietic disorders. The most important factors that influence the outcome of haematopoietic stem cell transplantations are the HLA match and the number of transplanted nucleated cells. The aim of our research was to analyse CB units donated to the Slovenian cord blood bank. Methods The concentrations of nucleated cells (NC) and haematopoietic stem cells (CD34+ cells) in CB were measured with a haematology analyser and flow cytometry. Growth of colonyforming cells (CFC) was determined in cell culture. The results were statistically evaluated using the Pearsons correlation analysis. Results The median NC concentration in CB was 10.6 × 10 9 /L (range: 4.07–26.2 × 10 9 /L), and the median number of NC was 721 × 10 6 (221–2157 × 10 6 ). The median collected volume of CB including 21 mL of anticoagulant was 73 mL (32–150 mL). The median concentrations of CD34+ cells and CFC were 35.5/μL (6.6–142.8/μL) and 17.7/μL (2.2–55.9/μL), respectively. The ratio of CD34+/CFC was 1.9 (range, 1.0–13.6), with a statistically significant positive correlation coefficient of 0.87. While there was a good correlation between the concentrations of NC and CD34+ cells, no correlation was observed between those concentrations and the collected volume of CB. Conclusions Of donated CB units, 46 % contained more than 750 × 10 6 nucleated cells and fulfilled the criteria for CB storage in our bank.Downloads
The Author transfers to the Publisher (Zdravniški vestnik/Slovenian Medical Journal) all economic copyrights following form Article 22 of the Slovene Copyright and Related Rights Act (ZASP), including the right of reproduction, the right of distribution, the rental right, the right of public performance, the right of public transmission, the right of public communication by means of phonograms and videograms, the right of public presentation, the right of broadcasting, the right of rebroadcasting, the right of secondary broadcasting, the right of communication to the public, the right of transformation, the right of audiovisual adaptation and all other rights of the author according to ZASP.
The aforementioned rights are transferred non-exclusively, for an unlimited number of editions, for the term of the statutory
The Author can make use of his work himself or transfer subjective rights to others only after 3 months from date of first publishing in the journal Zdravniški vestnik/Slovenian Medical Journal.
The Publisher (Zdravniški vestnik/Slovenian Medical Journal) has the right to transfer the rights, acquired parties without explicit consent of the Author.
The Author consents that the Article be published under the Creative Commons BY-NC 4.0 (attribution-non-commercial) or comparable licence.
