Does the prolonged in vitro maturation of human oocytes influence the aneuploidy type?
Abstract
Objective: In many stimulated infertility treatment cycles some oocytes are collected immature and can be matured in vitro (IVM). However, their safe clinical use is questionable because of their apparently low morphologic and genetic quality. We investigated the influence of the IVM duration on the type and frequency of chromosome abnormalities in germinal vesicle stage (GV) oocytes from stimulated cycles. Design: In-vitro maturation of GV oocytes (from stimulated cycles) for 24 (Group-A) or 36 (Group-B) hours and subsequent fluorescent in situ hybridization analysis (FISH) of chromosomes 13, 16, 18, 21 and 22. Results: After maturation, chromosomes were undoubtedly analyzable in 102 oocyte-first polar body pairs. Aneuploidy rates in both groups did not differ statistically. However, within Group-B a significantly higher rate of hyperhaploidy over hypohaploidy was observed. Also, there was a significantly higher frequency of disomy than nullisomy in both groups, and the aneuploidy rate of chromosomes 18 and 22 was significantly increased in Group-B. Conclusion: The observed preferential excess over the loss of genetic material and the increase in chromosome non-disjunction in the oocytes from Group-B, indicate a negative influence of prolonged IVM on chromosome segregation or conversely, suggests that GV oocytes which attain maturity later, possess more intrinsic abnormalities that result in aneuploidy.Design: In-vitro maturation of GV oocytes (from stimulated cycles) for 24 (Group-A) or 36 (Group-B) hours and subsequent fluorescent in situ hybridization analysis (FISH) of chromosomes 13, 16, 18, 21 and 22. Results: After maturation, chromosomes were undoubtedly analyzable in 102 oocyte-first polar body pairs. Aneuploidy rates in both groups did not differ statistically. However, within Group-B a significantly higher rate of hyperhaploidy over hypohaploidy was observed. Also, there was a significantly higher frequency of disomy than nullisomy in both groups, and the aneuploidy rate of chromosomes 18 and 22 was significantly increased in Group-B. Conclusion: The observed preferential excess over the loss of genetic material and the increase in chromosome non-disjunction in the oocytes from Group-B, indicate a negative influence of prolonged IVM on chromosome segregation or conversely, suggests that GV oocytes which attain maturity later, possess more intrinsic abnormalities that result in aneuploidy.Downloads
The Author transfers to the Publisher (Zdravniški vestnik/Slovenian Medical Journal) all economic copyrights following form Article 22 of the Slovene Copyright and Related Rights Act (ZASP), including the right of reproduction, the right of distribution, the rental right, the right of public performance, the right of public transmission, the right of public communication by means of phonograms and videograms, the right of public presentation, the right of broadcasting, the right of rebroadcasting, the right of secondary broadcasting, the right of communication to the public, the right of transformation, the right of audiovisual adaptation and all other rights of the author according to ZASP.
The aforementioned rights are transferred non-exclusively, for an unlimited number of editions, for the term of the statutory
The Author can make use of his work himself or transfer subjective rights to others only after 3 months from date of first publishing in the journal Zdravniški vestnik/Slovenian Medical Journal.
The Publisher (Zdravniški vestnik/Slovenian Medical Journal) has the right to transfer the rights, acquired parties without explicit consent of the Author.
The Author consents that the Article be published under the Creative Commons BY-NC 4.0 (attribution-non-commercial) or comparable licence.